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KMID : 0613820060160071141
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Journal of Life Science
2006 Volume.16 No. 7 p.1141 ~ p.1147
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Cytohistological Study of Development of Callus and Adventitious Shoots from Cultured Stem of Vigna radiata
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Park Jong-Bum
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Abstract
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This study was carried out to establish a reproducible culture system for callus formation and adventitious shoot development from young stem segments of Vigna radiata, and histological work for orgin of callus tissue and adventitious shoot. Induction of callus from young stem explants of Vigna radiata was very effective on MS inorganic salts supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L kinetin. For the adventitious shoot regeneration from the callus tissues, the hormone combination of 0.75 mg/L NAA, 1.5 mg/L kinetin and MS salts resulted in about 21% efficiency. Histological examination showed that callus tissues originated from out-growths by callus cambium rings with de novo meristematic activities, which were localized at the outside of the vascular cambium. Adventitious shoots were developed from shoot apical meristem originated from the surface of callus masses. The shoot apical meristem produced leaf primordium, which then became leaf.
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KEYWORD
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Vigna radiata, adventitious shoot, organogenesis, tissue culture
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